Abstract

Objective

In this study, it was aimed to investigate the protective effect of Ganoderma lucidum extract (GANO) in kidney toxicity developed with a contrast agent in rats who developed acute kidney injury with thirst and furosemide (diuretic) administration.

Methods: The rats were randomly grouped as follows: Sham, GANO200, RK (radiocontrast), RK+ISO, RK+GANO100, and RK+GANO200. Before administration of the contrast agent, rats were applied thirst and furosemide to develop acute kidney injury. GANO was given to the determined groups 1 hour before the contrast agent application. Afterward, RK (15 mL/kg) was administered from the tail vein of the rats. The kidney tissues were examined histopathologically and biochemically.

Results: In the Sham and GANO200 groups, the pathological finding was not observed in the glomerulus, proximal, and distal tubules. While shrinkage was observed in the renal corpuscular structures in the RK group, epithelial vacuolization was also seen in the proximal and distal tubules. Inflammatory cells were observed around the glomerular and distal tubules. The findings of the RK+GANO200 were found to be similar to the sham group. It was determined that the MDA level increased in the RK group, while the GSH level decreased. With GANO application, a decrease in MDA level and an increase in GSH level were observed. It was determined that serum creatinine and BUN levels increased in the radiocontrast agent applied groups and decreased after GANO application.

Conclusion: After contrast medium injury, G. lucidum extract application may play a protective role in renal tubules, especially in cases of acute kidney injury.

Cite this article as: Toktay E, Harmankaya A, Gül HF, et al. Protective effect of ganoderma lucidum extract in contrast agent induced renal toxicity. Arch Basic Clin Res 2022;4(2):74-80.