Abstract

Objective

The cellular protective effects of resveratrol and co-enzyme Q10 supplementation have been reported in different models. However, there is insufficient data on the effects of resveratrol supplementation on oxidative DNA damage and co-enzyme Q10 levels in ovarian ischemia–reperfusion injury. The current study aims to investigate how resveratrol supplementation affects oxidative DNA damage, co-enzyme Q10 levels, inflammatory and proinflammatory indicators, and growth factors in ovarian ischemia–reperfusion injury.

Methods

Thirty albino Wistar female rats were divided into 5 groups; control, ovarian ischemia–reperfusion, and ischemia–reperfusion+treatment (25, 50, and 100 mg/kg resveratrol). Hematoxylin–eosin stain was used to evaluate histopathological results. The levels of 8-oxo-2ʹ-deoxy guanosine and co-enzyme Q10 were obtained by enzyme-linked immunosorbent assay. Following RNA isolation, the expression of tumor necrosis factor-α , interleukin-1β, transforming growth factor-β3, hepatocyte growth factor, epidermal growth factor, and vascular endothelial growth factor was assessed.

Results

Regarding the histopathological evaluation score, 8-oxo-2ʹ-deoxyguanosine levels, and co-enzyme Q10 levels, there was no statistically significant difference between the ischemia–reperfusion group and the groups that received resveratrol treatment. Tumor necrosis factor-α, interleukin-1β, transforming growth factor-β3, hepatocyte growth factor, epidermal growth factor, and vascular endothelial growth factor expression levels were significantly decreased in the resveratrol -treated groups compared to the ischemia–reperfusion group.

Conclusion

The results imply that the positive effects of resveratrol on inflammatory-proinflammatory cytokine expression may be a major contributor to the anti-oxidant, anti-inflammatory, anti-proliferative, anti-carcinogenic, and therapeutic effects of resveratrol documented in the literature.

Cite this article as

Akbaş N, Gürbüzel M, Sayar İ, Bakan N. The effect of resveratrol on ischemia–reperfusion induced oxidative rat ovary injury: A biochemical, histopathological, and genetic evaluation. Arch Basic Clin Res., 2023;5(2):230-239.