Effect of Whitening Mouthrinses on Color Change of Stained Teeth: In Vitro Study

Objective: This in vitro study aimed to evaluate the effectiveness of 3 different whitening mouthrinses on the color change and whiteness of stained teeth at different immersion times.

Methods: Thirty-two human incisor teeth were used in the study. First, the teeth were stained in black tea solution for 7 days. Then, they were randomly divided into 4 groups, which were control (artificial saliva) and 3 whitening mouthrinses (containing tetrapo- tassium pyrophosphate, hydrogen peroxide, charcoal powder) (n=8). Spectrophotometric measurements were performed using a digital spectrophotometer at baseline, after staining, after 12 hours, and 120 hours after keeping in whitening mouthrinses. The color changes (ΔE00) and whiteness indexes (WID) occurring at different immersion times and after teeth staining were calculated. Data were analyzed using the generalized linear model method, Robust two-way analysis of variance, and post-hoc Tukey test (P < .05).

Results: The whitening mouthrinses tested showed similar changes in color while exhibiting color change above the clinically acceptable threshold (ΔE00 < 2.25). Regarding whiteness index values, the highest value was obtained in the mouthrinse containing hydrogen peroxide with an immersion time of 120 hours (P < .05). While there was no significant difference in color change values at different immersion times, it was observed that the whiteness index values increased with increasing immersion time.

Conclusion: Whitening mouthrinses can provide a clinically noticeable color change in discolored teeth. It can be said that using mouthrinses containing peroxide can provide more effective whitening on stained teeth.

Cite this article as: Gömleksiz S, Hubbezoğlu I. Effect of whitening mouthrinses on color change of stained teeth: in vitro study. Arch Basic Clin Res. 2025; Published online May 30, 2025. doi: 10.5152/ABCR.2025.24327.