Archives of Basic and Clinical Research
Original Article

Serum Amino Acid Levels in Children with Autism Spectrum Disorder by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry


Department of Medical Biochemistry, Amasya University, Faculty of Medicine, Amasya, Türkiye


Department of Medical Biochemistry, Atatürk University, Faculty of Medicine, Erzurum, Türkiye


Department of Medical Biochemistry, Erzincan Binali Yildirim University, Faculty of Medicine, Erzincan, Türkiye


Department of Nutrition and Dietetic, Atatürk University, Faculty of Health Sciences, Erzurum, Türkiye


Department of Nutrition and Dietetic, Lefke Europe University, Faculty of Health Sciences, Lefke, KKTC


Department of Child and Adolescent Psychiatry, Karadeniz Technical University, Trabzon, Türkiye


Departman of Child and Adolescent Mental Health, Kanuni Training and Research Hospital, Trabzon, Türkiye


Department of Medical Biochemistry, Üsküdar University, Faculty of Medicine, İstanbul, Türkiye

Arch Basic Clin Res 2023; 5: 208-217
DOI: 10.5152/ABCR.2023.221323
Read: 1349 Downloads: 535 Published: 02 February 2023

Objective: Autism spectrum disorder is defined by repetitive and restricted behaviors and lack of social interactions and communication. It is described as a neurodevelopmental disorder that appears early in life. Autism spectrum disorder was later noted as multifactorial disorder caused by environment, genetic, and other related factors. Amino acids are the precursors of neurotransmitters, and they are involved in the pathogenesis of several diseases and have many functions and effects in the body. They are indicators of protein metabolism and nutritional status. Because of its important properties and its potential relationship with autism, this study aimed to investigate serum amino acid levels in 20 healthy children and 25 children with autism spectrum disorder.

Methods: Serum amino acid levels were analyzed by liquid chromatography- mass spectrometry/mass spectrometry.

Results: Autism spectrum disorder group had a lower serum concentration of gamma-aminobutyric acid, aspartic acid, and beta-aminoisobutyric acid levels compared to the control group, while glutamic acid, tryptophan, alanine, glycine, proline, ornithine, histidine, and serotonin levels had statistically higher concentrations (P < .05). And also, serum levels of asparagine, arginine, methionine, serine, valine, tyrosine, threonine, lysine, leucine, beta alanine, anserine, hydroxyproline, argininosuccinate, isoleucine, sarcosine, citrulline, histamine, phenylalanine, and glutamine were not found to be statistically lower or higher (P > .05).

Conclusion: The study indicated that when compared to the control group, children with autism spectrum disorder had statistically significantly decreased and increased variation in some serum levels of amino acid.

Cite this article as: Gül MA, Umudum FZ, Kurt N,et al. Serum amino acid levels in children with autism spectrum disorder by liquid chromatography-mass spectrometry/mass spectrometry. Arch Basic Clin Res., 2023;5(1):208-217.

EISSN 2687-4482