The Relationship between the Presence of Helicobacter Pylori in the Normal Mucosa Adjacent to Tumor and the Degree of Differentiation in the Gastric Carcinoma. A Retrospective Analysis of 104 Gastrectomy Cases

Objective: To determine whether there is a relationship between the degree of differentiation of the tumor in patients with gastric carcinoma and Helicobacter pylori in the normal mucosa adjacent to the tumor.

Materials and Methods: 104 gastrectomy materials with carcinoma operated due to cancer at Istanbul Haseki Education and Research Hospital were examined retrospectively. Gastrectomy materials were fixed with 10% formalin. The paraffin blocks were prepared from tissue samples. 4 microns thick sections were taken from the blocks. The relationship between the presence of Helicobacter pylori in the mucosa adjacent to tumor and the degree of differentiation of gastric carcinoma was investigated. Descriptive summary statistics were obtained for all independent and outcome variables. Statistically significant p value was accepted to be less than 0.05. Statistical analysis was performed using SPSS statistical software (IBM SPSS Statistic for Windows, Version 20.0; IBM Corp., Armonk, NY, USA).

Results: Of the 104 carcinoma cases in the stomach, 5 were well-differentiated (4.8%), 20 were moderately differentiated (19.2%), 55 were low differentiated (52.9%), and 24 were undifferentiated (23.1%). Helicobacter pylori was found in 47 (45%) of 104 cases.  Helicobacter pylori was observed in 4 (4.8% ) of 5 well-differentiated carcinoma, in 13 (65%) of 20 moderately differentiated carcinomas, in 25 (45.4%) of 55 low-differentiated carcinomas, and in 5 of 24 undifferentiated carcinomas.

Conclusion: In parallel with the decrease in tumor differentiation in gastric carcinoma, the presence of Helicobacter pylori in normal mucosa adjacent to the tumor was decreased. Statistically significant difference was found (p<0.05).

Amaç: Mide karsinomu vakalarında tümörün diferansiasyon derecesi ile tümöre komşu normal mukozadaki Helicobacter pylori varlığı arasında ilişki olup olmadığını ortaya koymak.

Gereç ve Yöntem: İstanbul Haseki Eğitim ve Araştırma Hastanesinde kanser sebebiyle ameliyat edilen karsinom özelliğine sahip104 gastrektomi materyali retrospektif olarak incelendi. Gastrektomi materyalleri %10’luk formalin ile tesbit edildi. Doku örneklerinden parafin bloklar hazırlandı. Bloklardan 4 mikron kalınlığında kesitler alındı. Kesitler Hematoksilen-Eosin ve Giemsa ile boyandı. Mide karsinomunun diferansiasyon derecesi ile tümöre komşu normal mukozadaki Helicobacter pylori varlığı arasındaki ilişki incelendi. Tüm bağımsız ve sonuç değişkenleri için tanımlayıcı özet istatistikler elde edildi. İstatistiksel olarak anlamlı olan p değerinin 0,05’den küçük olduğu kabul edildi. İstatistiksel analiz SPSS istatistiksel yazılımı (Windows için IBM SPSS Statistic, Sürüm 20.0; IBM Corp., Armonk, NY, ABD) kullanılarak yapıldı.

Bulgular: Midedeki 104 karsinom vakasının 5’i iyi diferansiye (%4,8), 20’si orta diferansiye (%19,2), 55’i az diferansiye (%52,9), ve 24’ü indiferansiye (%23,1)idi. 104 vakanın 47’sinde (%45) Helicobacter pylori izlendi. 5 iyi diferansiye karsinomun 4’ünde (%80), 20 orta diferansiye karsinomun 13’ünde (%65), 55 az diferansiye karsinomun 25’inde (%45,4) ve 24 indiferansiye karsinomun 5’inde Helicobacter pylori (%20,8) izlendi.

Sonuç: Mide karsinomunda tümör diferansiasyon derecesinin azalmasına paralel şekilde tümöre komşu normal mukozada Helicobacter pylori varlığında azalma görüldü. İstatistiksel olarak anlamlı fark bulundu (p<0.05).

Cite this article as: Tayfur M, Balcı MG, Sınmaz Akalın G. The Relationship between the Presence of Helicobacter Pylori in the Normal Mucosa Adjacent to Tumor and the Degree of Differentiation in the Gastric Carcinoma. A Retrospective Analysis of 104 Gastrectomy Cases. Arch Basic Clin Res 2020; 2(1): 1-6.