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Objective: This study aimed to examine the effect of guanosine on reactive gliosis, neuroinflammation, and myelin loss in a kaolin-induced model of hydrocephalus.
Material and Method: Total 22 female rats were divided into the following 3 groups: 7 rats in Group 1 and Group 2 and 8 rats in Group 3. In Group 1 (sham), 30 μL of normal saline was injected into the cisterna magna. In Group 2 (hydrocephaly) and Group 3 (treatment) 30 μL of 25% kaolin suspension was injected into the cisterna magna. On the 14th day after the procedure, magnetic resonance imaging (MRI) was performed for identifying the hydrocephalus in Group 2 and Group 3. After the MRI, Group 2 and Group 3 received intraperitoneal vehicle and vehicle + guanosine injections for 7 days, respectively. On the 8th day, the animals were sacrificed under anesthesia. Histopathological, immunohistochemical, and immunofluorescent studies were performed, and statistical evaluation was performed with the Kruskal-Wallis and Mann-Whitney U tests.
Results: All the parameters examined in the hydrocephalic and treatment group were significantly different from those in the control group (p<0.005). However, there was no significant difference between the hydrocephalic and treatment groups in any parameter (luxol fast blue: p=0.867; IHC: p=0.55; IF: p=0.61).
Conclusion: In our study, guanosine did not have a significant effect on myelin loss and microglial activation in the hydrocephalus
model. However, for an effect to occur, a longer treatment period and administration of guanosine may be necessary during the development of hydrocephalus. More studies on this subject with guanosine or other protective agents are required.
Cite this article as: Yunusov R, Zırh EB, Kaymaz F, Bilginer B. Guanosine Has No Beneficial Effect on Reactive Gliosis, Neuroinflammation and Myelin Loss in the Experimental Rat Model of Hydrocephalus. Arch Basic Clin Res 2020; 2(2): 51-8.